rna polymerase

Listing 1 - 10 from 17 for rna polymerase

In situ hybridization of non-radiolabelled RNA probes to sectioned tissue.
... dig-UTP (Boehringer, cat#1209256) 5 microliters RNASIN (Promega) (RNAse inhibitor) to 1 unit/microliter RNA polymerase to 0.4 units/microliter water to total Total Volume 25 microliters Incubate the reaction ... minutes one 2.  Protease.  Tissue sections are proteased to increase the accessibility of the target RNA.  Treat sections with proteinase K (1 microgram/ml in 100 mM Tris (pH 8) and ...
carnegiedpb.stanford.edu

Brome mosaic virus
... available. Genome BMV contains 4 RNAs. RNA1 encodes P1 (methyltransferase and helicase), RNA2 encodes P2 (RNA polymerase), RNA3 encodes p3a (movement) and the capsid protein. RNA4 is a mRNA for the capsid ...
plantpath.unl.edu

Qβ replicase
... . 51, 225-70 (1995) Konarska, M.M., and Sharp, P.A. Replication of RNA by the DNA-dependent RNA polymerase of phage T7. Cell 57, 423-31 (1989) Su, Q., Schuppli, D., Tsui ... generating "parasitic" templates? 9- "Parasitic" small RNAs can infect T7 DNA dependent RNA polymerase VI- In vivo control of RNA synthesis 1- Ribosomes compete with replicase for binding 2- (+) strand is sequestered by ...
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On-Line Glossary: P
... and (c) the extension of the annealed primers with the heat insensitive Tag DNA polymerase. (10) polymorphism. Pleomorphism. polygenic. A character controlled by many genes. (20) polynucleate. Having more ... .) (20) promoter. A region of DNA, usually upstream of a coding sequence, that binds RNA polymerase and directs the enzyme to the correct transcriptional start site. (10) promycelium. The short ...
ppathw3.cals.cornell.edu

The Lac Operon
... for repressor Promoter (LacP) binding site for RNA polymerase Repressor (LacI) gene encoding lac repressor protein Binds to DNA at operator and blocks binding of RNA polymerase at promoter Pi promoter for LacI CAP ... and binds to the DNA, so that RNA polymerase can no longer get past the promoter. No RNA and no protein is made. Note that RNA polymerase can still bind to the promoter though it ...
web.mit.edu

Other Regulated Systems
... . The sequence of this RNA allows it to form a terminator loop only when ribosomes are bound to it, at which point the RNA is cleaved and the RNA polymerase stops transcribing the genes ... protein called luxR. This protein somehow calls RNA polymerase over when it is bound to the DNA, thus increasing transcription of the DNA and making mre polymerase. Thus, luxR is a transcriptional activator ...
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Biologie Cellulaire : Equipes
... history acces contact research groups publications Keywords :Arabidopsis thaliana - Mutant -Chromatin -Methylation - RNA interference - siRNA - miRNA -Development - Virus Doctoral school affiliation : Contacts : Hervé Vaucheret, ... producing trans-acting siRNAs. Curr Biol 15, 1494-1500 (PubMed) Vaucheret (2005) RNA polymerase IV and transcriptional silencing. Nat Gen 37, 659-660 (PubMed) Park, Wu, ...
www-ijpb.versailles.inra.fr

Graphics Gallery
... Intron Repressor Protein Switching Genes On and Off RNA - (ribonucleic acid) RNA - A More Detailed View Initiation of Transcription by RNA Polymerase mRNA (Messenger RNA) Control of the Human B-Globin Gene ... Transfer and Cloning of the Insulin Gene Cloning into a YAC Monoclonal Antibody Production Polymerase Chain Reaction Transgenic Mice Cell Breakage and Fractionation Part 1 Cell Breakage and ...
www.accessexcellence.org

LaMarco/Regulation of Gene Expression
... for RNA polymerase and the general transcription factors. In eukaryotic cells, RNA Polymerase II (RNAPII; a large complex of proteins whose enzymatic activity does the actual copying of DNA into RNA ... trancriptional regulatory proteins enhance transcription in the context of chromatin. Abbreviation Key: RNAPII - RNA polymerase II HSV - Herpes simplex virus IE genes - HSV immediate early genes GABP - ...
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APSnet Education Center - Introductory Topics - What are Fungicides?
... of sterols or chitin. For example, phenylamide fungicides bind to and inhibit the function of RNA polymerase in oomycetes, while the benzimidazole fungicides inhibit the formation of beta tubulin polymers used by ...
www.apsnet.org

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