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SDS gel electrophoresis - advice for beginners
... reduced proteins reoxidize with time. Freezing does not inhibit reoxidation. I prefer to reduce with dithiothreitol (DTT, a stronger reducing agent) and then alkylate proteins with iodoacetamide to stabilize them in the ...
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Stabilizing and "resurrecting" DTT (dithiothreitol) stock solutions
... DTT (dithiothreitol) stock solutions Home Gel electrophoresis tips Precipitating proteins and nucleic acids Reduction alkylation protocol Dithiothreitol (DTT) solutions Structure of dithiothreitol, DTT History of DTT Stabilizing and "resurrecting" DTT (dithiothreitol) stock solutions Ensure your DTT ... and the oxygen volume as low as possible. "Good" and "bad" DTT compared. Dithiothreitol (DTT ...
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