potato dextrose agar

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The Blue Plate Test
... The bromophenol blue plates are petri plates which contain potato dextrose agar (PDA), a microbial growth medium. The agar causes the media to gel and the potato dextrose (sugar) provides a nutrient source for the ... fields. If they are present, they will produce a type of colony on the potato dextrose agar that can be distinguished from that of the white mold pathogen. Field Sampling Procedure ...
plantpath.unl.edu

FAQ - Fungi and Mycorrhizae
... binary fission. Figure 2.: Thread-like mycelium in a colony of Rhizoctonia solani growing on potato dextrose agar. This fungus causes brown patch disease of turfgrass and damping-off in seedlings of many ...
res2.agr.gc.ca

OREP - Use of natural fruit volatiles for reduction of postharvest rot of stonefruits and pears
... volatiles for Penicillium expansum were evaluated for their potential to inhibit mycelium actively growing on Potato Dextrose Agar. In general, higher concentrations were required for control of P. expansum. These screening trials identified ...
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APSnet Education Center - Lab Exercises in Plant Pathology - Following the disease progression of an ectotrophic root-infecting fungus.
... strength potato dextrose agar 7-10 days prior to use. This ensures the plates are completely colonized and actively growing. Using good aseptic technique, cut agar ... borer. Two plugs will be needed per student. Agar disks can be cut into the agar up one day in advance of the lab. ... 's Note #4, 5). Preparation of agar tubes. Prepare 1 liter of 0.3% water agar (3 g agar per liter) per 45 tubes to ...
www.apsnet.org

New Disease Reports - First report of Rhizoctonia solani causing leaf spot of Adhatoda vasica
... infection resulted in defoliation. Repeated isolations from infected leaves yielded a similar fungal growth on Potato Dextrose Agar. Five-day-old cultures (26 ± 1°C) produced profuse mycelium and dirty white sclerotia. Older ...
www.bspp.org.uk

New Disease Reports - First report of Verticillium wilt of melon caused by Verticillium dahliae in Tunisia
... of fungi were consistently and readily isolated from symptomatic stem vascular tissue when cultured on potato dextrose agar (PDA) medium. A Verticillium species was the only fungus isolated and it grew from most ...
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Brown Rot/Calcium
... except calcium formate, calcium pantothenate, and dibasic calcium phosphate reduced fungal growth on amended potato dextrose agar (PDA) after 7 days. Minimal growth occurred on PDA amended with calcium propionate. ... % of the control. Substances that were inhibitory in amended PDA also were inhibitory in potato dextrose broth (PDB), but varied in effectiveness depending on whether PDB or PDA was used ...
www.caf.wvu.edu

Isolation of Soil Microorganisms
... using aseptic techniques. Materials: 1 Erlenmeyer flask containing 50ml of sterile agar (0.1%) 1 cup containing 0.5g of soil 4 small ... of sterile agar (0.1%) 5 sterile 1ml pipettes 1 sterile glass stirring rod 6 Petri plates containing about 10ml of PDA (potato dextrose agar) 6 ... Add the 0.5g soil to the Erlenmeyer flask containing 50ml of agar (this is the 10-2 dilution). Shake well for at least ...
www.cps-scp.ca

Isolement des microorganismes du sol
... de travail aseptique. Matériel: 1 flacon Erlenmeyer contenant 50 ml d’agar (0,1%) stérile 1 nacelle contenant 0,5 g de ... agar (0,1%) stérile 5 pipettes stériles de 1 ml 1 bâtonnet de verre stérile ("hockey") 6 plats de Petri contenant environ 10 ml du milieu de culture PDA (Potato dextrose agar ... et déposer dans une fiole contenant 4,5 ml d’agar 0,1 %. (Ceci est la dilution 10-3). Agiter pendant au ...
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How to Identify and Control Dogwood Anthracnose
... on dead twig. Discula sp. conidia. Cultural Characteristics The fungus grows slowly on malt or potato dextrose agar. Colonies are appressed, granular and white, and darken with age. Sporulation of Discula sp. in ...
www.na.fs.fed.us

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